3D Printing in the Donnelly Lab
How useful can 3D printing be in a lab setting? Here’s what I’ve found
I first started off with 3D printing (3DP) a few years ago with the Anet A8 (A8). The A8 is a cheap build-it-yourself type kit, which does the job of 3D printing objects, albeit not at the greatest resolution straight out of the box.
I have spent numerous hours upgrading, tweaking, fixing and generally tinkering with the A8 and now have it in a condition where it reliably produces prints analogous to the commercially available and professionally calibrated 3D printers, specifically, the Original Prusa i3 MK2 which the A8 is a clone of.
Left: Prusa MK2. Right: Anet A8.
The most common question I get when people find out I have a 3D printer is either how much did it cost? or what do you use 3DP for?
To answer the how much? question I’m digging deep to remember, but it was roughly £120, and now checking GearBest.com the current price (on 04/10/2018) is £108, so £120 seems about right.
The why question is a bit trickier to directly answer. But generally, I enjoy tinkering with things and building things that I need, and 3DP is a great technology for getting very specific, precise and intricate parts for a build project. I had found myself saying I could 3D print that long before deciding to buy one. But, the final time was when I was putting my tool wall back together after moving, and deciding that printing dedicated tool holders would be extremely useful, for organising small parts and hand tools.
So, after searching for a long time, I decided on the A8 (a printer which I do not recommend buying, for what it’s worth), ordered it, it arrived, I assembled it and began printing. Now, after many hours of print-time, repair-time, upgrading-time, and finding-tiny-screws-that-I-dropped-onto-the-carpet-time, I’m starting to explore more complex areas of 3DP technologies.
Printing in the lab
I had seen that some people on Thingiverse had designed custom items for specific needs within their lab, ranging from bespoke Eppendorf tube holders to centrifuge tube adapters to rocker trays. However, I never really thought of suggesting we try and use 3DP in our lab, as I didn’t think there was a need for it. That was until Dr Arjen Van ‘T Hof mentioned that he had been looking into 3DP as a method for maximising storage space in the BioBank that the Donnelly lab manages. After demo-ing my printer for a week in the lab, the Donnelly group ordered its very own printer.
The Donnelly lab 3D printer.
-80°C BioBank Tube Storage
For some context on this, a typical 9x9 storage box for Eppendorf tubes is designed for 1.5ml tubes. This means that when you have 0.5 ml tubes stored in these boxes, they are more difficult to remove and liable to being bounced from their place and could cause tube mix-up. Dr Van ‘T Hof had the idea of designing custom tube storage boxes that still retained the outer format of the traditional 9x9 boxes, but which had more appropriate dividers to accommodate the smaller tubes, whilst also increasing the number of tubes per box. After some tweaking and experimenting, Dr Van ‘T Hof had designed the model for the tube box and began printing them to fulfil the BioBank’s storage needs.
-20°C Pre-PCR Primer Tube Storage
Additionally, aliquots of qPCR primers which need to be stored in separate boxes, quickly fill freezer space in the PCR-prep room. At any one time, a primer pair has ~15 aliquots, so we designed a set of boxes to efficiently hold those 15 tubes, to maximise the freezer storage space. We also added unique labels to both parts of the box, to prevent mix up between sample boxes.
Dr Van ‘T Hof also mentioned an idea for printing a gel electrophoresis casting comb that had 1 more tooth than usual. This is because typical combs have multiples of 8 teeth, meaning that you are unable to do multiple complete rows of 8 (relevant because of PCR tray layout) and have a ladder lane. I initially intended to make my own 3D model for this, but why reinvent the wheel? A quick search on Thingiverse revealed that Shyam Bhakta has already designed a parametric version of the electrophoresis gel comb. We customised the design to fit our needs, added a label, and began printing.
Ice Block for SDS-PAGE Tanks
Dr Lee Haines had heard about the 3DP that was going on and asked whether it could solve the problem of replacing parts no longer available from the manufacturer or are available only in limited supply and as a result, unreasonably priced. The SDS-PAGE system in the lab uses an ice block to keep the buffer cool, whilst the experiment is running. The ice block is an irregularly shaped plastic ‘bowl’ which is filled with water and put in the freezer. The frozen block is then used for an experiment run and then refrozen. At this point, Dr Van ‘T Hof was quite adept with CAD and pulled together the 3D model for this part and we began printing. The first version was a success (with the exception of the colour choice, we later found out).
Hopefully, by now I’ve convinced you that 3DP has some useful applications in a lab setting, when you want to either design a bespoke item or replace parts that are difficult and/or expensive to acquire. In addition to these, there are also small, more miscellaneous, yet still handy items that have been printed around the lab. Below are some pictures.
Small Parts Storage in a 50ml tube
Tube holder for DNA Extraction
Multichannel Pipette Holder
Micropestle Wash Bucket
Current 3DP Projects
Long Print Projects
At the moment I’m working to complete AKSHAY_D21’s 3D printable laboratory rocker. This is more of a personal project so I can play around with the Arduino framework, with the added benefit that the end product actually has a use.
Dr Van ‘T Hof and I are working to design .STL models of the WHO Insecticide Susceptibility Bioassay tube systems, to help replace broken parts in the insectaries and keep as many tube kits as possible in operation. This is pushing the limits of my CAD skills, but with the help of Dr Van ‘T Hof, who has recently figured out thread creation, we should be able to complete this project. I intend to write a separate blog post about this in the near future when we have substantial progress.
Have An Idea for a Print?
If you’re an LSTM colleague and have an idea for a print that could help out around the lab/office, please get in touch.
Please feel free to leave feedback on the blog post, either with Disqus below or my email above.